Weissella: An Emerging Bacterium with Promising Health Benefits. Journal of Applied Polymer Science, 27, 795-810. 2019;26(1):25261. Keywords: WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Webof overall macromolecular geometry and hydrodynamic draining. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. Proc of SPIE. Int J Pharm Investig. Dextrans can function efficiently as anterograde or retrograde tracers, ; Ross-Murphy, S.B. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. ; Fran, H.P. Journal of Chemical Education, 80(9), 1036-1038. Biomaterials Research The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. All branched polysaccharide types could be described approximately by the hyperbranching theory. To confirm the extent of skin damage and regeneration after microporation, porations were performed 2, 5, and 10 times. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. Ind. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Biomaterials, 22, 1891-1898. Huggins equation [41] gives the relation to the relative viscosity increase (i). Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). 2013, Article ID 360239, pp. 2015;32(5):170413. Dynamics of a Polymer Solution in a Rigid Matriz. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Ubbelohde, Leo. Journal of Physical Chemistry. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. Journal Argentine Chemical Society 100 (2013) 25-34. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Kumar SV, Tarun P, Kumar TA. 2013;43(4):287303. In addition, the permeation rate was higher in pig skin than in human skin. Hennink, W.E. Format), Citation-(EndNote We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. 2012;101(8):28619. Pure Appl. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. They concluded that a power law behavior seems to be approached only in the small molecular weight region. Arond L.H. Chen Rong Huei; Min Lang Tsai (1998). Structure Properties of Dextran. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". FITC-dextran solution (600L) was added to the donor chamber. The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. The micropore formed at the SC through to the epidermis, enabling drug delivery. Progr. Formation of dextran hydrogels by crystallization. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Dextran Fractions. ; Picout, D.R. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Smith, David R.; Raymonda, John W. (1972). Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). Monolayers and Thin Films of Dextran Hydrophobically Modified. Pharm Res. A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Macromolecules, 33, 5730-5739. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. Synthesis and characterization of blue dextrans. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. The slope is a value and intercept is equal to the ln kMH value [47]. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Microporation was performed 2 times on each sample. 2005;22(4):5505. (2003). Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Terms and Conditions, Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. 2012;9(2):14754. Eng. Elias, Hans-Georg (1975). Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. (1999). Critical parameters for gel permeation The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Naik A, Kalia YN, Guy RH. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Banga AK. Accueil; Solution; Tarif; PRO; Mon compte; France; Accueil; Solution; Tarif; PRO; Mon compte In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Carbohydrate Polymers, 38, 267-271. High Manufacturing Standards of Molecular Probes Dextrans. Effect of temperature on the intrinsic viscosity and conformation of chitosan in dilute HCl solution. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. (2006). Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. (1958). Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Global hydrodynamic analysis of the molecular flexibility of galactomannans. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Gold MH, Biron JA. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. ; Yevlampieva N.P. (2001). The human skins were from the back or thigh of a 24-year-old woman. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). GYA primarily conducted research. Intrinsic viscosity-molecular weight relationship for chitosan. Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. Treatment of acne scars by fractional bipolar radiofrequency energy. TEWL measurements were taken 30min after microporation of the human and pig skin. Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Walkenhorst, S.; Olivier, R. (2002). 1994, 95-0355. of different origins. Gekko, K. (1971). (2007). Provided by the Springer Nature SharedIt content-sharing initiative. and transmitted securely. The hydrodynamic radius of 2-NBDG glucose was too small to measure. European. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should dextran hydrodynamic radius. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. If there are particles in the sample, the incident laser light gets scattered in all directions. Carbohydrate Polymers, 68, 477-488. Polysaccharides-based nanoparticles as drug delivery systems. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. Rheology of dextran solutions. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Masuelli, Martin Alberto (2013). Masuelli, Martin Alberto. Molecular weight determination of polysaccharides. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth The solution was measured using an Anton Paar DMA35N densitometer. Harding, S. E., & Simpkin, N. J. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Figure4 shows the histological analysis of micropores in human cadaver skin. 2011;5(2):95132. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. However, the currently available transdermal systems are limited by low drug permeability across the skin due to the lipophilic barrier function of the stratum corneum (SC), the outermost skin layer of the skin [3,4,5]; the methods favor low molecular mass, and lipophilic molecules, making it difficult to exploit the transdermal route for large and water-soluble molecules such as proteins and peptides. Chem. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). osmotic pressure or light scattering). Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. Porcine wound models for skin substitution and burn treatment. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. Progress in Biophysical Molecules Biological, 68, 207-262. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. Therefore, it is suitable as model molecules with different molecular weights for transdermal drug delivery [29]. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. a Immediately after microporation, b 4h, c 8h, and d 24h (n=5, *: p<0.05). Journal of Applied Polymer Science, 72, 871-876. Chromatographic characterization of polymers: Hyphenated and multidimensional techniques. Pharm Res. Differences were considered statistically significant at p <0.05. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. NCI CPTC Antibody Characterization Program. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. ; Weisz, J. 2010;4(1):117. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Im Tiefkhlgert (-5 bis -30C) lagern und vor Licht schtzen. International Journal of Biological Macromolecules, 23, 135-141. European Polymer Journal, 42, 1086-1092. Chem. Advances in Cosmetic Surgery. Privacy 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. These vibrations heat the skin tissue, triggering water vapor and cell ablation, forming microchannels from the SC through to the outer dermis. (2007). Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. The mechanism of the dextran-induced red blood cell Aggregation. Hindered diffusion of dextran and ficoll in microporous membranes. Exp Cell Res. The Mark-Houwink a constant is close to 0.5 in poor solvents. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Our portfolio includes for example derivatives of dextran, polysucrose, inulin and more. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. The flattening of the curves at higher molecular weight is in agreement with an increased branching density. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Radiofrequency ablation of malignant liver tumors. Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). (2003). DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. Karmarkar, S.; Garber, R.: Kluza, J.; Koberda, M. (2006). Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. From Table 1, It is worth noting that the value of 2 is above 0.95, being an acceptable value for viscosimetric measurement. Data File Dextran. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Microporation techniques for enhanced delivery of therapeutic agents. 2019;9(3-S):9726. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. Kasaai, Mohammad R. (2012). Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. International Journal of Biological Macromolecules, 42, 133-137. European Polymer Journal, 43, 1744-1753. Learn More. Increased TEWL values are evidence of micropores formation. 4K, b M.W. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). The hydrodynamic 57 radius of the dextranase is 7 4K and M.W. Ibrahim O, Munavalli GS, Dover JS. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. II. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Google Scholar. Physicochemical studies of oligodextran. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. 4h after microporation, the sizes of the micropore decreased slightly. The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , The human and pig skin permeability of FITC-dextran (M.W. 113, 81-86. 2000;3(9):31826. Journal of American Chemical Society, 76 (20), 5041-5052. 2008;28(5):64557. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. Unable to load your collection due to an error, Unable to load your delegates due to an error. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Polymolecularity and polydispersity in Molecular weight determinations. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv.
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